Part:BBa_K1969004

GAL1 promoter

This is the full length of yeast GAL1 promoter cloned from genomic DNA of Saccharomyces cerevisiae. In the range of 1-118 bp of the promoter, there is an upstream activating sequence mediating GAL4-dependent induction. All the galactose structural genes (GAL1, GAL10, GAL7, GAL2) are coordinately regulated at the level of transcription in response to galactose by Gal4p, Gal80p, and Gal3p. Regardless of carbon source, the Gal4p transcriptional activator is bound as a dimer to upstream activation sites found in the promoters of the GAL genes. In the presence of galactose, Gal3p sequesters the transcriptional repressor Gal80p in the cytoplasm, thereby relieving inhibition of Gal4p and resulting in GAL gene expression.

Contribution from other teams

Toulouse_INSA-UPS 2021's contribution

Characterization

Toulouse_INSA_UPS_2021contributed to the characterization of this part. The part BBa_K1969004 for GAL1 promoter coming from S. cerevisiae, inducible by the addition of galactose in the medium, had not been characterized before in iGEM, but the team showed this year that it is functional for expression in S. cerevisiae. To do this the promoter was placed upon the LcyE-ofCCD1 gene, allowing the production of α-ionone and this molecule was identified thanks to GC-MS approaches. Check the part of the full construction (BBa_K3930003) for more result details.

(--ThomasG 16:53, 18 October 2021 (UTC+2))

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 70
  
• 1000
  COMPATIBLE WITH RFC[1000]